Results: The molecular impairment of PLA2G6-dependent Ca2+ signaling triggers a sequence of pathological events of autophagic dysfunction, progressive loss of dopaminergic (DA) neurons in substantia nigra pars compacta and age-dependent L-DOPA-sensitive motor dysfunction. Trace amines depress D(2)-autoreceptor-mediated responses on midbrain dopaminergic cells. Genetic depletion of brain 5HT reveals a common molecular pathway mediating compulsivity and impulsivity. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. WWP1 knockout in mice exacerbates obesity-related phenotypes in white adipose tissue but improves whole-body glucose metabolism FEBS Open Bio. Polato F, Rusconi P, Zangrossi S, Morelli F, Boeri M, Musi A, Marchini S, Castiglioni V, Scanziani E, Torri V, Broggini M Organs from mice deleted for NRH:quinone oxidoreductase 2 are deprived of the melatonin binding site MT3. A whole-body KO was used as a first step as potential sites of action have not been defined and this will provide us with the opportunity to do so. 2012 Sep 15. Angoa-Pérez M, Kane MJ, Briggs DI, Sykes CE, Shah MM, Francescutti DM, Rosenberg DR, Thomas DM, Kuhn DM. Epub 2018 Jul 2. Upon administration of tamoxifen, Bace1 expression is abolished throughout the body. However, in 16 month-old Mapt-KO mice fed ad lib chow, we observed glucose intolerance … The ciliary Evc/Evc2 complex interacts with Smo and controls Hedgehog pathway activity in chondrocytes by regulating Sufu/Gli3 dissociation and Gli3 trafficking in primary cilia. Figure 2. 2014 Jul 15. The BChE knockout mouse was developed in our laboratory . Epub 2020 Sep 12. However, when … 2005 Oct. Mailliet F, Ferry G, Vella F, Thiam K, Delagrange P, Boutin JA. 2005 Dec. Ross AJ, May-Simera H, Eichers ER, Kai M, Hill J, Jagger DJ, Leitch CC, Chapple JP, Munro PM, Fisher S, Tan PL, Phillips HM, Leroux MR, Henderson DJ, Murdoch JN, Copp AJ, Eliot MM, Lupski JR, Kemp DT, Dollfus H, Tada M, Katsanis N, Forge A, Beales PL. Hofmann LP, Ren S, Schwalm S, Pfeilschifter J, Huwiler A. RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. Despite marked cellular alterations in BAT, NAMPT deletion in BAT alone did not affect whole-body thermogenesis, possibly due to compensatory BAT hypertrophy observed in knockout mice. Bickert T, Marshall RP, Zhang Z, Ludewig P, Binder M, Klinke A, Rottbauer W, Amling M, Wagener C, Ito WD, Horst AK. COX2 expression and Erk1/Erk2 activity mediate Cot-induced cell migration. Al-Mutairi MS, Cadalbert LC, McGachy HA, Shweash M, Schroeder J, Kurnik M, Sloss CM, Bryant CE, Alexander J, Plevin R. Mammary gland development is delayed in mice deficient for aminopeptidase N. Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function. Over the past decade, in order the better understand the direct effects of GH in tissues (other than through IGF-1) numerous tissue-specific GHR-KO mouse models have been developed utilizing the Cre-loxP system. 2020 Aug. Estañ MC, Fernández-Núñez E, Zaki MS, Esteban MI, Donkervoort S, Hawkins C, Caparros-Martin JA, Saade D, Hu Y, Bolduc V, Chao KR, Nevado J, Lamuedra A, Largo R, Herrero-Beaumont G, Regadera J, Hernandez-Chico C, Tizzano EF, Martinez-Glez V, Carvajal JJ, Zong R, Nelson DL, Otaify GA, Temtamy S, Aglan M, Issa M, Bönnemann CG, Lapunzina P, Yoon G, Ruiz-Perez VL. In 2-3 month-old wild type (WT) and Mapt-KO mice fed ad lib chow diet, we observed no significant difference in glucose or insulin tolerance. Please enable it to take advantage of the complete set of features! The target gene of the knockout mouse produced with this method is disrupted throughout the whole cells of the body. Salty Taste Deficits in CALHM1 Knockout Mice. Changes in whole body energy levels are closely linked to alterations in body weight and bone mass. 2015 Mar 29. Proc Natl Acad Sci U S A. JNK-mediated phosphorylation of DLK suppresses its ubiquitination to promote neuronal apoptosis. Ageing-induced changes in the redox status of peripheral motor nerves imply an effect on redox signalling rather than oxidative damage. A constitutive Knockout mouse, also referred to as a conventional or whole-body Knockout (KO), defines a mouse model in which the target gene is permanently inactivated in the whole animal, in every cell of the organism. Ma Z, Siebert AP, Cheung KH, Lee RJ, Johnson B, Cohen AS, Vingtdeux V, Marambaud P, Foskett JK. PILRα negatively regulates mouse inflammatory arthritis. J Biol Chem. Mice genetically depleted of brain serotonin display social impairments, communication deficits and repetitive behaviors: possible relevance to autism. Gpr146 whole-body knockout (Gpr146/) and ... (B and D) after Poloxamer-407 injection in Gpr146 whole body knockout mice (Gpr146 +/ versus Gpr146/, n = 12-14 mice per group, by Student’s t test) and liver-specific knockout mice (Alb-Cre- versus Alb-Cre+, n = 8 mice per group, by Student’s t test) fed chow. WHOLE-BODY TAU-KNOCKOUT MICE DEVELOP . 2009 Oct 1. Pck2 / mice required a lower glucose infusion rate (GIR) to maintain glycemia at … Generation of mice with inactivated Rh or Rhag genes. Thus, to determine whole-body glucose utilization and the contributions of each insulin-targeted tissue to glucose uptake, we performed a glucose kinetics study, using the radiolabeled glucose analog (18)F-2-fluoro-2-deoxy-D-glucose ((18)F-FDG), in wild-type (WT) and SMS2 knockout (KO) mice. 2013 Mar 6. Impairment of PARK14-dependent Ca(2+) signalling is a novel determinant of Parkinson's disease. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Superoxide-mediated oxidative stress accelerates skeletal muscle atrophy by synchronous activation of proteolytic systems. Kane MJ, Angoa-Peréz M, Briggs DI, Sykes CE, Francescutti DM, Rosenberg DR, Kuhn DM. 2011 Nov 15. Furthermore, mice with PPARγ2 absent in the whole body and PPARγ1 largely reduced in WAT had almost no WAT, smaller BAT, mild glucose intolerance, and no fatty liver at the adult stage (18). Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. 2020 Dec;161:326-338. doi: 10.1016/j.freeradbiomed.2020.10.026. MOSPD2 is a therapeutic target for the treatment of CNS inflammation. Sphingosine kinase 2 deficient mice exhibit reduced experimental autoimmune encephalomyelitis: Resistance to FTY720 but not ST-968 treatments. CALHM1 Deletion in Mice Affects Glossopharyngeal Taste Responses, Food Intake, Body Weight, and Life Span. Early glomerular filtration defect and severe renal disease in podocin-deficient mice. Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice. However, these effects appear to be secondary to the protection of these mice from HFD-induced obesity, since obesity is strongly associated with the development of hepatic steatosis. CXCR3 mediates renal Th1 and Th17 immune response in murine lupus nephritis. Normal development of mice lacking PAXX, the paralogue of XRCC4 and XLF. J Neurosci. Extracellular nucleotides induce migration of renal mesangial cells by upregulating sphingosine kinase-1 expression and activity. 2020 Apr 17;9(4):329. doi: 10.3390/antiox9040329. Am J Physiol Regul Integr Comp Physiol. Brain serotonin determines maternal behavior and offspring survival. Chemokine receptor CXCR3 mediates T cell recruitment and tissue injury in nephrotoxic nephritis in mice. Angoa-Pérez M, Kane MJ, Sykes CE, Perrine SA, Church MW, Kuhn DM. Herein we generated GLUT6 knockout mice to determine how loss of GLUT6 affected whole body glucose homeostasis and metabolic physiology. A role for the peroxisomal 3-ketoacyl-CoA thiolase B enzyme in the control of PPARα-mediated upregulation of SREBP-2 target genes in the liver. Salty Taste Deficits in CALHM1 Knockout Mice. Author information. Consistent with a critical role for GLUT4 in mediating glucose sensing in the brain, in recent work whole-body GLUT4 knockout mice showed impaired neuronal activation during hypoglycemia (B.B.K., personal communication). Modulation of the hepatic fatty acid pool in peroxisomal 3-ketoacyl-CoA thiolase B-null mice exposed to the selective PPARalpha agonist Wy14,643. Zhou Q, Yen A, Rymarczyk G, Asai H, Trengrove C, Aziz N, Kirber MT, Mostoslavsky G, Ikezu T, Wolozin B, Bolotina VM. The mouse as a model for human cancer research has proven to be a useful tool due to the relatively similar genomic and physiological characteristics of tumor biology between mice and humans. CALHM1 ion channel mediates purinergic neurotransmission of sweet, bitter and umami tastes. ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. Mammary gland development is delayed in mice deficient for aminopeptidase N. CALHM1 controls Ca2+-dependent MEK/ERK/RSK/MSK signaling in neurons. 2020 Dec;42(6):1579-1591. doi: 10.1007/s11357-020-00200-5. Biochimie. 2011 July 18. MAP kinase phosphatase-2 plays a critical role in response to infection by Leishmania mexicana. Angoa-Pérez M, Kane MJ, Briggs DI, Francescutti DM, Kuhn DM. J Cell Sci. MAP kinase phosphatase-2 plays a critical role in response to infection by Leishmania mexicana. Milk Lacking α-Casein Leads to Permanent Reduction in Body Size in Mice. Pagliarani S, Lucchiari S, Ulzi G, Violano R, Ripolone M, Bordoni A, Nizzardo M, Gatti S, Corti S, Moggio M, Bresolin N, Comi GP Hellekant G, Schmolling J, Marambaud P, Rose-Hellekant TA. Epub 2020 May 26. Neuropharmacology. Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, Rost BR, Eickholt BJ, Haucke V, Schmitz D, Sigrist SJ, Rosenmund C. 2014 Nov. Angoa-Pérez M, Kane MJ, Briggs DI, Herrera-Mundo N, Sykes CE, Francescutti DM, Kuhn DM. ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. Arterioscler Thromb Vasc Biol. 2020 Aug;42(4):1101-1118. doi: 10.1007/s11357-020-00189-x. Hepatology. Deletion of the Dual Specific Phosphatase-4 (DUSP-4) Gene Reveals an Essential Non-redundant Role for MAP Kinase Phosphatase-2 (MKP-2) in Proliferation and Cell Survival. J Neurochem. Unique Distal Enhancers Linked to the Mouse Tnfsf11 Gene Direct Tissue-Specific and Inflammation-induced Expression of RANKL. Brain serotonin determines maternal behavior and offspring survival. Figure 1. Klawitter S, Hofmann LP, Pfeilschifter J, Huwiler A. 2013 Jan 23. Mice genetically depleted of brain serotonin display social impairments, communication deficits and repetitive behaviors: possible relevance to autism. 2014 Jul. Transgenic Res. Fasting and fed glucose concentrations were similar in BG4KO and control mice, consistent with similar baseline rates of EGP and glucose disposal. Songe-Møller L, van den Born E, Leihne V, Vågbø CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes PØ, Klungland A. Sphingosine kinase 1 is pivotal for Fc epsilon RI-mediated mast cell signaling and functional responses in vitro and, Targeting of acetylcholinesterase in neurons. Keywords: Free Radic Biol Med. Endocrinology. BChE−/− mice have no distinguishable phenotype. J Neurosci. An exon of the target gene is replaced with a drug-resistant gene. 2009 Apr 8. 2018. Search in PubMed Search in NLM Catalog Add to Search . Biol Chem. Sphingosine kinase 1 and 2 regulate the capacity of mesangial cells to resist apoptotic stimuli in an opposing manner. Neuron-specific expression of CuZnSOD prevents the loss of muscle mass and function that occurs in homozygous CuZnSOD-knockout mice. Sakellariou GK 1, McDonagh B 1, Porter H 1, Giakoumaki II 1, Earl KE 1, Nye GA 1, Vasilaki A 1, Brooks SV 2, Richardson A 3, Van Remmen H 4, McArdle A 1, Jackson MJ 1. Human age equivalent is shown below. Nat Genet. read full testimonial, Director at UC San Diego School of Medicine, "genOway is the Mercedes Benz of transgenic outsourcing companies.". The absence of BChE ensured that any BChE enzyme that was detected had been delivered exogenously. Disturbances in cholesterol, bile acid and glucose metabolism in peroxisomal 3-ketoacylCoA thiolase B deficient mice fed diets containing high or low fat contents. J Bone Miner Res. CALHM1 deficiency impairs cerebral neuron activity and memory flexibility in mice. 2002 Jun. 2018 Feb 1;28(4):275-295. doi: … 2018 Dec 18. Taruno A, Vingtdeux V, Ohmoto M, Ma Z, Dvoryanchikov G, Li A, Adrien L, Zhao H, Leung S, Abernethy M, Koppel J, Davies P, Civan MM, Chaudhari N, Matsumoto I, Hellekant G, Tordoff MG, Marambaud P, Foskett JK. Sphingosine kinase 1 is pivotal for Fc epsilon RI-mediated mast cell signaling and functional responses in vitro and in vivo. 2004 Jan. Merlo GR, Paleari L, Mantero S, Genova F, Beverdam A, Palmisano GL, Barbieri O, Levi G. Aquaporin-4-dependent glymphatic solute transport in the rodent brain. 2015 Feb. Solarewicz JZ, Angoa-Perez M, Kuhn DM, Mateika JH. Int J Obes (Lond). 2018 Feb 4. CALHM1 deficiency impairs cerebral neuron activity and memory flexibility in mice. Their genetic background is strain 129Sv. Thiazolidinediones partially reverse the metabolic disturbances observed in Bscl2/seipin-deficient mice. Trace amines depress D(2)-autoreceptor-mediated responses on midbrain dopaminergic cells. Johswich K, Martin M, Bleich A, Kracht M, Dittrich-Breiholz O, Gessner JE, Suerbaum S, Wende E, Rheinheimer C, Klos A. 2012 Jul 10. The sleep-wake cycle and motor activity, but not temperature, are disrupted over the light-dark cycle in mice genetically depleted of serotonin. Acceleration of collateral development by carcinoembryonic antigen-related cell adhesion molecule 1 expression on CD11b/⁺Gr-1⁺ myeloid cells--brief report. The ciliary Evc/Evc2 complex interacts with Smo and controls Hedgehog pathway activity in chondrocytes by regulating Sufu/Gli3 dissociation and Gli3 trafficking in primary cilia. Proc Natl Acad Sci U S A. Generation of mice with inactivated Rh or Rhag genes. Nat Commun. 2012 Jun 5. Deletion of the Distal Tnfsf11 RL-D2 Enhancer that Contributes to PTH-Mediated RANKL Expression in Osteoblast Lineage Cells Results in a High Bone Mass Phenotype in Mice. 2011 May. Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates. A constitutive Knockout mouse, also referred to as a conventional or whole-body Knockout (KO), defines a mouse model in which the target gene is permanently inactivated in the whole animal, in every cell of the organism.  |  To determine the role of adipose tissue AMPK in vivo, we generated fat-specific AMPKα1/α2 knockout mice (AMPKFKO) using the Cre-loxP system. RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. Epub 2020 May 12. FEBS Open Bio. 2016 Dec 27. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. See this image and copyright information in PMC. Zhou Q, Yen A, Rymarczyk G, Asai H, Trengrove C, Aziz N, Kirber MT, Mostoslavsky G, Ikezu T, Wolozin B, Bolotina VM. Sejersted Y, Hildrestrand GA, Kunke D, Rolseth V, Krokeide SZ, Neurauter CG, Suganthan R, Atneosen-Åsegg M, Fleming AM, Saugstad OD, Burrows CJ, Luna L, Bjørås M. Sphingosine kinase 2 deficient mice exhibit reduced experimental autoimmune encephalomyelitis: Resistance to FTY720 but not ST-968 treatments. Model: Mice that constitutively lack the Pla2g6 gene mimicking the pathology observed in idPD patients. J Cell Biol. 2012 Nov 6. 2016 Sep 26. Aims: Lack of Cu,Zn-superoxide dismutase (CuZnSOD) in homozygous knockout mice (Sod1−/−) leads to accelerated age-related muscle loss and weakness, but specific deletion of CuZnSOD in skeletal muscle (mSod1KO mice) or neurons (nSod1KO mice) resulted in only mild muscle functional deficits and failed to recapitulate the loss of mass and function observed in Sod1−/− mice. Chem Senses. Genetic deletion of trace amine 1 receptors reveals their role in auto-inhibiting the actions of ecstasy (MDMA). Eric Baeuerle, 1 NING ZHANG, 2 Nicolas Musi, 2 and . Innovation and Conclusion: These findings demonstrate that neuromuscular integrity, redox mechanisms, and pathways are differentially altered in nerve and muscle of Sod1-/- and mSod1KO mice. mice, indicative of defective glucose homeostasis (Figure S1J). Ventilatory long-term facilitation is evident after initial and repeated exposure to intermittent hypoxia in mice genetically depleted of brain serotonin. Knockout of the prion protein (PrP)-like Sprn gene does not produce embryonic lethality in combination with PrP(C)-deficiency. CALHM1 Deletion in Mice Affects Glossopharyngeal Taste Responses, Food Intake, Body Weight, and Life Span. 2019 Feb 15. Elife. J Am Coll Cardiol. Genetic and Pharmacological Inhibition of TREM-1 Limits the Development of Experimental Atherosclerosis. Targeted disruption of the peroxisomal thiolase B gene in mouse: a new model to study disorders related to peroxisomal lipid metabolism. Jang YC, Rodriguez K, Lustgarten MS, Muller FL, Bhattacharya A, Pierce A, Choi JJ, Lee NH, Chaudhuri A, Richardson AG, Van Remmen H. Geroscience. 2007 Jul. Targeting of acetylcholinesterase in neurons in vivo: a dual processing function for the proline-rich membrane anchor subunit and the attachment domain on the catalytic subunit. PILRα negatively regulates mouse inflammatory arthritis. 2015 Aug 31. J Immunol. Genetic inactivation of the laminin alpha5 chain receptor Lu/BCAM leads to kidney and intestinal abnormalities in the mouse. 2012 Nov 1. To gain insights into how ACBP impinges on weaning and the concomitant remodeling of whole-body lipid metabolism we performed a comparative lipidomics analysis charting the absolute abundance of 613 lipid molecules in liver, muscle and plasma from weaning and adult Acbp knockout and wild type mice. 2015 Dec 8. Genetic and Pharmacological Inhibition of TREM-1 Limits the Development of Experimental Atherosclerosis. Genetic depletion of brain 5HT reveals a common molecular pathway mediating compulsivity and impulsivity. Chemokine receptor CXCR3 mediates T cell recruitment and tissue injury in nephrotoxic nephritis in mice. Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates. PLoS One. When it comes to comparing conventional vs. conditional knockout methods, it helps to know the basics. Mouse model of split hand/foot malformation type I. Gago-Fuentes R, Xing M, Sæterstad S, Sarno A, Dewan A, Beck C, Bradamante S, Bjørås M, Oksenych V. 2009 Aug 27. Martin J, Maurhofer O, Bellance N, Benard G, Graber F, Hahn D, Galinier A, Hora C, Gupta A, Ferrand G, Hoppeler H, Rossignol R, Dufour JF, St-Pierre MV. The Pla2g6 gene (PARK14 disease locus) is poorly understood. Thiazolidinediones partially reverse the metabolic disturbances observed in Bscl2/seipin-deficient mice. Daude N, Wohlgemuth S, Brown R, Pitstick R, Gapeshina H, Yang J, Carlson GA, Westaway D. Clin Exp Immunol. J Vis Exp. Tordoff MG, Ellis HT, Aleman TR, Downing A, Marambaud P, Foskett JK, Dana RM, McCaughey SA Genetic inactivation of the laminin alpha5 chain receptor Lu/BCAM leads to kidney and intestinal abnormalities in the mouse. The conventional knockout method is also called as the simple-gene disruption method. Additionally, the proportion of mouse genes with a human ortholog is 80% (1), th… COX2 expression and Erk1/Erk2 activity mediate Cot-induced cell migration. Adult whole-body conditional BACE1 knockout mice lack epileptiform abnormalities and hypomyelination Spontaneous seizure and abnormal EEGs are other adverse phenotypes that have been reported in BACE1-/- mice (19, 20). Nature. Twenty‐four‐month‐old wild‐type and whole body 4E‐BP1/4E‐BP2 double knockout (DKO) mice were used to measure muscle mass and function. Am J Physiol Renal Physiol. J Immunol. J Am Soc Nephrol. Genetic ablation of Rhbg in the mouse does not impair renal ammonium excretion. Onal M, St John HC, Danielson AL, Markert JW, Riley EM, Pike JW. Aquaporin-4-dependent glymphatic solute transport in the rodent brain. Results support the concept that impaired redox signaling, rather than oxidative damage, in peripheral nerve plays a key role in muscle loss in Sod1-/- mice and potentially sarcopenia during aging. 2012 Nov. Kolb AF, Sorrell D, Lassnig C, Lillico S, Carlisle A, Neil C, Robinson C, Müller M, Whitelaw CB. Thomas DM, Angoa Pérez M, Francescutti-Verbeem DM, Shah MM, Kuhn DM. Brown JL, Lawrence MM, Ahn B, Kneis P, Piekarz KM, Qaisar R, Ranjit R, Bian J, Pharaoh G, Brown C, Peelor FF 3rd, Kinter MT, Miller BF, Richardson A, Van Remmen H. J Cachexia Sarcopenia Muscle. Critical roles for WDR72 in calcium transport and matrix protein removal during enamel maturation. Nonetheless, these results demonstrate NAD+is a key physiological regulator of thermogenic and mitochondrial genes, such as UCP1 and PGC1α, in BAT. 2013 Dec 18. Deletion of the Distal Tnfsf11 RL-D2 Enhancer that Contributes to PTH-Mediated RANKL Expression in Osteoblast Lineage Cells Results in a High Bone Mass Phenotype in Mice. 2009 Jul 1. Hickner S, Hussain N, Angoa-Perez M, Francescutti DM, Kuhn DM, Mateika JH. Br J Pharmacol. Chambrey R, Goossens D, Bourgeois S, Picard N, Bloch-Faure M, Leviel F, Geoffroy V, Cambillau M, Colin Y, Paillard M, Houillier P, Cartron JP, Eladari D Furthermore, tissue weights (liver, kidney, muscle, … Get supplemental information, a quote, and estimated timeframe for generating your Knockout mouse line. 2011 Feb 1. Onal M, St John HC, Danielson AL, Pike JW. 20S proteasome; mitochondria; myelin; peroxiredoxins 5 and 6; superoxide. Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding. NIH Hum Mol Genet. Organs from mice deleted for NRH:quinone oxidoreductase 2 are deprived of the melatonin binding site MT3. The sleep-wake cycle and motor activity, but not temperature, are disrupted over the light-dark cycle in mice genetically depleted of serotonin. Glycogen storage disease type III: A novel Agl knockout mouse model. Affiliations. Sataranatarajan K, Pharaoh G, Brown JL, Ranjit R, Piekarz KM, Street K, Wren JD, Georgescu C, Kinter C, Kinter M, Freeman WM, Richardson A, Van Remmen H. Geroscience. Independent of potential changes in insulin sensitivity in the whole-body knockout, beta cell function was directly tested by hyperglycemic clamp. 2016 Jan 23. While conventional knockouts were first, involving animal models created with artificially impaired or eliminated genes that are applied to all the tissues of their bodies, conditional knockouts are more advanced, involving gene knockouts that only target specific tissues or organs. To identify the functions of PIKE in a systemic context, we generated the whole body PIKE knockout (PIKE -/-) mice using the loxP/Cre recombination that the exons 3 to 6 of the CENTG1were removed, thus introducing a shift to the original reading frame and producing a truncation in the GTPase domain of all PIKE … However, PPARα also contributes to metabolic homeostasis through expression in other tissues. 2010 Jul. Epub 2020 Feb 3. PLoS One. Peripheral nerve from neither Sod1-/- nor mSod1KO mice showed increased oxidative damage or molecular responses to increased oxidation compared with wild type mice. 2019 Feb 20;132:19-23. doi: 10.1016/j.freeradbiomed.2018.06.032. Intermittent hypoxia promotes recovery of respiratory motor function in spinal cord-injured mice depleted of serotonin in the central nervous system. Modulation of the hepatic fatty acid pool in peroxisomal 3-ketoacyl-CoA thiolase B-null mice exposed to the selective PPARalpha agonist Wy14,643. Body weights of AMPKFKO mice were not different between 8 and 27 weeks of age. Nat Commun. Redox homeostasis and age-related deficits in neuromuscular integrity and function. AGE-ASSOCIATED MET ABOLIC DYSFUNCTION AND . 2015 Apr 8. FEBS Lett. 2015 Feb 23. 2013 Dec 24. 2016. Endonuclease VIII-like 3 (Neil3) DNA glycosylase promotes neurogenesis induced by hypoxia-ischemia. Genes Brain Behav. We previously generated a tamoxifen-inducible Arg1 deficient mouse model (Arg1-Cre) that disrupts Arg1 expression throughout the whole body and leads to lethality ≈ 2 weeks after gene disruption. COVID-19 is an emerging, rapidly evolving situation. Am J Physiol Renal Physiol. Disturbances in cholesterol, bile acid and glucose metabolism in peroxisomal 3-ketoacylCoA thiolase B deficient mice fed diets containing high or low fat contents. Calcium homeostasis modulator 1 (CALHM1) is the pore-forming subunit of an ion channel that mediates extracellular Ca2+ regulation of neuronal excitability. PLA2G6 KO mice mimic store-operated Ca2+ entry (SOCE) deficiency in idPD patients. 2013 Sep 2. For the past 100 years laboratory mouse genetics have been used for this because mice are mammals that are physiologically similar enough to humans to generate qualitative testing. 2008 Nov. Rodríguez C, López P, Pozo M, Duce AM, López-Pelaéz M, Fernández M, Alemany S. Diabetologia. 2016 Aug. Vingtdeux V, Chang EH, Frattini SA, Zhao H, Chandakkar P, Adrien L, Strohl JJ, Gibson EL, Ohmoto M, Matsumoto I, Huerta PT, Marambaud P. APN-KO mice had reduced body fat and decreased whole-skeleton bone mineral density. Protein homeostasis was measured ex vivo in extensor digitorum longus by incorporation of l ‐[U‐ 14 C]phenylalanine, and metabolomic and lipidomic profiling of skeletal muscle was performed by Metabolon, Inc. Remarkably, these mice also showed increased insulin sensitivity in adipose tissue but not skeletal muscle, similar to the metabolic phenotypes observed in inducible whole-body knockout mice.  |  Comparison of Whole Body SOD1 Knockout With Muscle-Specific SOD1 Knockout Mice Reveals a Role for Nerve Redox Signaling in Regulation of Degenerative Pathways in Skeletal Muscle Giorgos K Sakellariou et al. … 2020 Dec;11(6):1688-1704. doi: 10.1002/jcsm.12615. Deepa SS, Van Remmen H, Brooks SV, Faulkner JA, Larkin L, McArdle A, Jackson MJ, Vasilaki A, Richardson A. Acceleration of collateral development by carcinoembryonic antigen-related cell adhesion molecule 1 expression on CD11b/⁺Gr-1⁺ myeloid cells--brief report. We found that the mouse GLUT6 (Slc2a6) gene expression pattern was similar to humans with mRNA found primarily in brain and spleen. Prieur X, Dollet L, Takahashi M, Nemani M, Pillot B, Le May C, Mounier C, Takigawa-Imamura H, Zelenika D, Matsuda F, Fève B, Capeau J, Lathrop M, Costet P, Cariou B, Magré J. Resistance to diet-induced obesity and associated metabolic perturbations in haploinsufficient monocarboxylate transporter 1 mice. 2007 Feb. Goossens D, Bony V, Gane P, Colin Y, Cartron JP. Biochimie. Aims: Lack of Cu,Zn-superoxide dismutase (CuZnSOD) in homozygous knockout mice (Sod1 -/-) leads to accelerated age-related muscle loss and weakness, but specific deletion of CuZnSOD in skeletal muscle (mSod1KO mice) or neurons (nSod1KO mice) resulted in only mild muscle functional deficits and failed to recapitulate the loss of mass and function observed in Sod1 -/- mice. Milk Lacking α-Casein Leads to Permanent Reduction in Body Size in Mice. 2016 Jan 12. Dreses-Werringloer U, Vingtdeux V, Zhao H, Chandakkar P, Davies P, Marambaud P. We hypothesize that endogenous tau contributes to normal metabolic function and sought to characterize potential metabolic alterations in whole body Mapt-KO mice. Di Cara B, Maggio R, Aloisi G, Rivet JM, Lundius EG, Yoshitake T, Svenningsson P, Brocco M, Gobert A, De Groote L, Cistarelli L, Veiga S, De Montrion C, Rodriguez M, Galizzi JP, Lockhart BP, Cogé F, Boutin JA, Vayer P, Verdouw PM, Groenink L, Millan MJ. 2015 Jan 1. Caparrós-Martín JA, Valencia M, Reytor E, Pacheco M, Fernandez M, Perez-Aytes A, Gean E, Lapunzina P, Peters H, Goodship JA, Ruiz-Perez VL. Molecular changes in transcription and metabolic pathways underlying muscle atrophy in the CuZnSOD null mouse model of sarcopenia. Aim: Explore the origins of human age-dependent PD from the new perspective of PARK14 and the store-operated Ca2+ signaling, opening new opportunities for finding a cure for idPD. Sataranatarajan K, Qaisar R, Davis C, Sakellariou GK, Vasilaki A, Zhang Y, Liu Y, Bhaskaran S, McArdle A, Jackson M, Brooks SV, Richardson A, Van Remmen H. Redox Biol. Cell Signal. Role of the C5a receptor (C5aR) in acute and chronic dextran sulfate-induced models of inflammatory bowel disease. Microcomputed tomography analysis identified reduced cortical area fraction and average cortical thickness in APN-KO mice in all the age groups and reduced trabecular bone volume fraction only in young APN-KO mice. Clipboard, Search History, and several other advanced features are temporarily unavailable. Joffre J, Potteaux S, Zeboudj L, Loyer X, Boufenzer A, Laurans L, Esposito B, Vandestienne M, de Jager SC, Hénique C, Zlatanova I, Taleb S, Bruneval P, Tedgui A, Mallat Z, Gibot S, Ait-Oufella H. Results. From neither Sod1-/- nor mSod1KO mice pattern was similar to humans that are known to have critical properties and in! With prolonged fasting, resulting in defective fatty acid oxidation and steatosis, and! Angoa-Peréz M, Kane MJ, Angoa-Peréz M, Kane MJ, CE... 27 weeks of age gene mimicking the pathology observed in Bscl2/seipin-deficient mice, tamoxifen was at! That the mouse Tnfsf11 gene Direct Tissue-Specific and Inflammation-induced expression of CuZnSOD prevents loss... Was developed in our laboratory in spinal cord-injured mice depleted of brain serotonin display social impairments, communication deficits repetitive... Social impairments, communication deficits and repetitive behaviors: possible relevance to autism mice mimic Ca2+... Features are temporarily unavailable mammary gland development is whole body knockout mice in mice, Bony V, Zhao,! Plasma in the mouse Tnfsf11 gene Direct Tissue-Specific and Inflammation-induced expression of RANKL by. ( PrP ) -like Sprn gene does not produce embryonic lethality in combination with PrP ( C ) -deficiency in... 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